BioOncology Watch

Timely Information for Practicing Physicians

 

OCTOBER 2000

Non-Hodgkin's Lymphoma (NHL)

Rituximab retreatment.  Treatment of patients with low-grade or follicular NHL with single-agent rituximab (IDEC Pharmaceuticals Corp), a chimeric anti-CD20 monoclonal antibody, has previously been shown to result in an overall response rate (ORR) of 48% and a median time to progression (TTP) of 13.2 months.  Thomas Davis and associates conducted a phase II study in which 58 patients with low-grade or follicular NHL who had relapsed after responding to rituximab therapy were retreated with rituximab (375 mg/m2 I.V. weekly x 4).  The median interval between rituximab courses was 14.5 months (range, 3.8 to 35.6 months).  The ORR was 40% and the Kaplan-Meier estimated median TTP was 17.8 months (range, 3.7+ to 26.6 months).  No patient developed human anti-chimeric antibodies and hematologic toxicity was generally mild and reversible.  This study shows that retreatment of patients with low-grade or follicular NHL with rituximab is effective and is not associated with cumulative bone marrow suppression.  (Davis TA, et al. J Clin Oncol 2000;18:3135-3143)

 

Long-term follow-up after radioimmunotherapy (RIT).  Mark Kaminski and colleagues report long-term data (up to 8 years) on 59 B-cell NHL patients following a single treatment with CD20-targeted RIT (iodine 131I tositumomab; Coulter Pharmaceutical, Inc) at the University of Michigan. Forty-two patients (71%) achieved a response and the median progression-free survival for responders was 12 months.  Sixteen patients were retreated after disease progression and 9 responded (5 CRs).  Late adverse events included 5 patients who developed secondary myelodysplasia (MDS) 1.2 to 7.5 years after RIT.  The MDS evolved to acute myeloid leukemia in one patient.  These patients had received a median of 4 prior chemotherapy regimens and all had previously been exposed to alkylating agents.  An additional 3 patients were diagnosed with solid tumors following RIT.  Five patients developed elevated thyroid-stimulating hormone levels, but none were symptomatic.  These data show that RIT can be effective therapy for patients with advanced NHL.  Further studies are needed to fully assess the risk for secondary malignancies. (Kaminski MS, et al. Blood 2000; 96:1259-1266)

 

Allogeneic Stem-Cell Transplantation

Therapy for renal-cell carcinoma.  Renal-cell carcinoma responds to immunomodulatory therapies and Richard Childs et al. postulated that a graft-versus-tumor effect might also be generated by non-myeloablative bone marrow transplantation.  A pilot study was performed in which 19 patients with refractory metastatic renal-cell carcinoma received a preparative regimen of cyclophosphamide and fludarabine followed by an infusion of a peripheral-blood stem-cell allograft from an HLA identical sibling or a sibling with a mismatch of a single antigen.  Cyclosporine therapy was used to prevent graft-versus-host disease and patients with no initial response received up to 3 infusions of donor lymphocytes.  Ten patients (53%) achieved a response, which developed only after all T-cells in the recipient were of donor origin (complete chimerism).  Thus, the onset of tumor regression occurred at a median of 4 months (range, 1 to 8 months) after transplantation.  In 8 patients tumor regression was not observed until after the cyclosporine had been withdrawn.  These preliminary results demonstrate that allogeneic T-cells can survive following non-myeloablative conditioning and induce antitumor activity in patients with refractory advanced renal-cell carcinoma. (Childs R, et al. N Eng J Med 2000;343:750-758)

 

Donor lymphocyte infusion (DLI) therapy for relapsed multiple myeloma.  H. M. Lokhorst and coworkers administered DLI treatments to 27 patients with relapsed multiple myeloma after allogeneic stem-cell transplantation.  Thirteen patients received reinduction chemotherapy and 8 patients responded partially.  Fourteen of the 27 patients (52%) achieved a response (6 CRs and 8 PRs) to DLI treatments.  In 5 patients the response was induced following T-cell dose escalation in subsequent DLIs.  Five patients remained in remission for more than 30 months after DLI and the median survival for all patients was 18 months.  Factors that were identified as predictors for response to DLI were chemotherapy-sensitive disease prior to transplantation, response to reinduction chemotherapy, and a T-cell dose >1 x 108 cells/kg. Acute and chronic graft-versus-host disease occurred in 15 (56%) and 7 (26%) patients, respectively, and 2 patients died from bone marrow aplasia.  DLI is effective treatment for patients with relapsed multiple myeloma after allogeneic stem-cell transplantation and may induce long-term remissions in some patients.  (Lokhorst HM, et al. J Clin Oncol 2000;18:3031-3037)

 

Leukemia

Gemtuzumab zogamicin.  K. Naito and colleagues investigated the mechanism of action of gemtuzumab zogamicin (CMA-676; Wyeth Lederle, Japan), a calicheamicin-conjugated humanized anti-CD33 murine monoclonal antibody, in several myeloid leukemia cell lines.  A selective cytotoxic effect was seen in cell lines that expressed CD33.  The cytotoxic effect was dependent on the gemtuzumab zogamicin dose, the degree of CD33 expression, and the rate of proliferation of the leukemic cells.  P-gp-expressing multidrug-resistant sublines were refractory to gemtuzumab zogamicin.  In the presence of MDR modifiers (eg. MS209 and PSC833) these resistant leukemic cells became sensitive to the cytotoxic effects of gemtuzumab zogamicin.  The combination of gemtuzumab zogamicin and MDR modifiers is a potential therapeutic modality for patients with multidrug-resistant acute myeloid leukemia.  (Naito K, et al. Leukemia 2000;14:1436-1443)

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